Large chromatic variations between I, M and C areas of the same tooth were registered ( Δ E a b * = 5.01–6.07 and Δ E 00 = 4.07–5.03 Δ E a b * = 5.80–8.16 and Δ E 00 = 4.37–5.15 and Δ E a b * = 5.42–5.92 and Δ E 00 = 3.87–4.16 between C and M, C and I and M and I, respectively). Mean color differences between UCI and ULI exceeded AT ( Δ E a b * = 7.39–7.42 Δ E 00 = 5.71–5.74) in all cases. Non-perceptible color differences were found between UCIs and ULIs. Δ E a b * and Δ E 00 total color differences were computed between different tooth areas and adjacent teeth, and evaluated according to the perceptibility (PT) and acceptability (AT) thresholds for dentistry. CIE-L*a*b* values were calculated for the incisal (I), middle (M) and cervical (C) third of each target tooth. Hyperspectral reflectance images (Specim IQ), of the upper central (UCI) and lateral incisors (ULI), were captured on 30 participants. The aim of this study was to use a hyperspectral imaging system for the colorimetric characterization of 4 in-vivo maxillary anterior teeth and to cross-check the results with similar studies carried out with other measuring systems in order to validate the proposed capturing protocol. In this sense, hyperspectral imaging can provide point-wise reliable measurements of the tooth surface, which can serve for this purpose. A full comprehension of colorimetric relationships within and between teeth is key for aesthetic success of a dental restoration.
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